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Figure 1

The figure shows the impact of GuSCN concentration on the recovery of yeast THI4 mRNA. Yeast THI4 mRNA was captured at increasing GuSCN concentrations ranging from 0 to 4 M GuSCN using a DNA-T20 probe or a LNA™-T20 probe, and electrophoresed in a 1 % agarose gel.

Figure 2

The figure shows the recovery (%) of the yeast THI4 mRNA by the DNA-T20 probe and the LNA™-T20 probes (yellow bars) at increasing GuSCN concentrations.

Figure 3

The figure shows the recovery (%) of polyadenylated yeast ACT1 spike mRNA in 4 M guanidinium thiocyanate buffer at different temperatures using the DNA-T20 probe and the LNA™-T20 probe (yellow bars).

mRNA Isolation

Efficient poly(A)+ RNA isolation using LNA™ enhanced oligo-T20 capture probe
  • Get efficient isolation of intact poly(A)+RNA under the harshest conditions.
  • Poly(A)+RNA isolation from tissue and cell extracts in 4M GuSCN extraction buffer
  • The high content of GuSCN inactivates nucleases and proteases and dissociates proteins from your target mRNA
  • Increased stringency during capture at elevated temperatures secures improved access to target
  • 50-fold higher recovery of poly(A)+RNA
  • Compatible with beads, tubes and microtiter plate formats
  • Fully developed protocols


For further information, view the material under Documentation to the right.


Product

Order LNA™ mRNA sample prep (5'-amino)
Order LNA™ mRNA sample prep (5'-biotin)
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Documentation

Publications

Jacobsen et al. Nucleic Acid Res., 2004, 32, e64

Technical notes

Efficient poly(A)+ RNA selection (477 KB)
mRNA isolation from biological material (77 KB)
mRNA isolation from total RNA (74 KB)


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