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| miRCURY LNA microRNA knockdown library for genome-wide screening of microRNA function
At a glance
- Tm normalized probes specially designed for high throughput screening
- Coverage of all human and mouse microRNAs in miRBase v. 12.0
- Practical 96-well plate format for high throughput screening
Coverage
Human library
This library targets all 866 human microRNAs listed in miRBase v. 12.0. In addition, the library targets 36 human proprietary miRPlus™ microRNAs not yet in miRBase – in total 902 human microRNA.
In addition it is possible to order KD probes for all human virus encoded microRNA listed in miRBase v. 12.0 as well as 399 human miRPlus™ microRNA not yet in miRBase. For more information please enquire and see paragraph below on miRPlus™ microRNA
Mouse library
This library targets all 627 mouse microRNA listed in miRBase v.12.0.
In addition it is possible to order KD probes for all mouse virus encoded mircroRNA listed in miRBase 12.0. For more information please enquire.
Combined Human and mouse library
308 probes that target phylogenetically conserved microRNA have been organized in separate 96 well plates (plates 6 – 9) that are part of both the human and mouse libraries. This allows you to purchase a combined human and mouse library without being obliged to buy these 308 probes twice. For example it is possible to extend the human library to comprise all mouse probes by purchasing the 316 probes targeting mouse microRNAs not covered by the human library.
In the plate layout files (human, mouse) you find a list of all microRNAs covered by the libraries.
Designed for genome-wide high throughput screening
The miRCURY LNA™ microRNA Knockdown libraries enable genome-wide high throughput screening of microRNA function. The knockdown probes in these libraries have been specially designed for this purpose.
With large screening projects involving hundreds of knockdown probes uniform transfection conditions must be adopted for reasons of practicality i.e. the same amount of each probe in the library is used for all the transfections. For this reason it is crucial that all the knockdown probes have similar potencies towards their intended microRNA target.
This is not the case with libraries based on traditional antisense inhibitors (for example 2’-OMe oligonucleotides) since the potency of such inhibitors is dictated to a large extent by the GC content of their target microRNA.
We have solved this problem by carefully designing the length, sequence and LNA™ spiking patterns of our knockdown probes. Uniform high potency of all the microRNA inhibitors in the library was accomplished by ensuring Tm normalization around an optimal temperature while keeping the level of self-complementarity to a minimum.
High potency and purity minimizes side effects
Due to the high potency of novel designed knockdown probes they can be used efficiently at low concentrations. This minimizes problems with secondary effects, not related to antisense inhibition of the microRNA target, which may confound the phenotypic readout from the cellular assay.
The probes are HPLC purified and desalted after purification. The high purity of the probes ensures minimal side effects from toxic byproducts.
Format
The libraries are delivered in 96-well plates. Each well contains 0.25 nmol dried down miRCURY LNA knockdown probe. Plate layouts are available as downloadable Excel files – Human, Mouse
miRPlus™ - proprietary human microRNAs
miRCURY LNA™ arrays have since 2006 featured capture probes for proprietary microRNA “miRPlus™” sequences not yet in miRBase. Over the years, various versions of our arrays have featured capture probes for 274 different miRPlus™ microRNA. Out of these sequences, 236 are today annotated in miRBase v. 12.0, which means that almost 30% of the currently annotated human microRNAs were available on our arrays at some point before they were included in miRBase.
Recently we have increased our content of human miRPlus sequences to no less than 435 novel microRNA, not yet in miRBase, identified by deep sequencing of human normal and diseased tissue. The chromosomal loci of the corresponding pre-mir sequences have been mapped. Our latest version of microarray features capture probes for all 435 miRPlus™ microRNA and figure 1 shows differential expression of these microRNA in six different tissues.
Now we also offer KD probes for all these novel microRNAs of unknown function. In addition to the 36 miRPlus already included in our normal human library it is also possible to order knockdown probes for the remaining 399 miRPlus™ microRNAs in our database. For more information please contact us.
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| For prices and ordering please contact us.
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